2D-VisiFRAP and VisiFRAP DC

Fluorescence Recovery After Photobleaching and Full Range Photo-Manipulation

2D-VisiFRAP Realtime Scanner

Fluorescent dyes show emission of one wavelength after they have absorbed light of short wavelength. Dyes, exposed to high intensity e.g. UV light react by photobleaching. The high intense light  renders the dyes unable to emit fluorescence. Fluorescence Recovery is based on this phenomenon and is typically used to measure the dynamics of molecular mobility or movement of fluorescent labeled molecules. It is also possible to measure the exchange of molecules between separate compartments of the cells.

The 2D-VisiFRAP system from Visitron Systems GmbH is a microscope based imaging solution specially designed for fluorescence recovery or photoactivated studies. The system is based on a 2D galvanometer realtime scanner, a high sensitive CCD camera system, a research microscope like  Zeiss AxioObserver, Olympus BX/IX or Nikon E2000.
The 2D galvanometer scanner head is typically mounted on the epi fluorescence condenser. The laser light can be simple adapted via standard FC-connector. Customer requirements can be easily integrated due to the flexibility of the system. Realtime multi-point confocal technology can be easily combined to enhance resolution- and image quality.
Typically 405nm, 430nm, 457nm or 488nm laser-systems are used for bleaching multiple point or multiple area positioning is selectable by the new VS MetaImaging Software module which allow the positioning of the laser beam by two high speed galvanometer. Fast switching of the laser lines is done by the high speed VS-AOTF100 system or high speed shutter. The powerful and easy to use Meta-Imaging Software control the microscope and peripherals in combination with the camera system.

2D-VisiFRAP-DC System with integrated laser

Full Range Photo-Manipulation Imaging System
New: - from 355nm to VIS Laser – NO limit with laser lines

  • With unlimited number and size of regions
  • Auto-calibration

The latest model extension of the Visitron System 2D-FRAP scanner family convinces by its compactness and flexibility. The new design allows the direct coupling of up to two lasers in the Galvo-scan-head without laser fibres. An additional laser fibre input offers the combination of the system with other VIS-laser lines if required.
This design allows the easy combination of UV laser (355nm / 405nm) used e.g. in ablation experiments, together with VIS laser for FRAP or other photomanipulations of the specimen. This unique new scan head opens new dimension in biological studies of living cells.

The 2D-VisiFRAP-DC System is based on a pair of galvanometer-driven scan mirrors directly computer controlled by the VisiView imaging software. The Scan head is mounted and coupled to the FL-excitation port of the microscope, where it directly manipulate the specimen.

 

FRAP on the fly
The optimized system components allow simultaneous FRAP and imaging at single mouse
click on any position in the live image. This new feature in the VisiView FRAP software is
minimising any loss of temporal information and by taking advantage of the flexibility and high
speed positioning of the VS-FRAP scanner. The unique “FRAP on the fly” solves perfectly the
major demand for photo-manipulation experiments.
 

Auto-Calibration
With the automatic signal and spot detection of our VisiView imaging software, the autocalibration
algorithm calibrates the FRAP scanner. It shows the laser spot and the accuracy of
the calibration in several regions on the display. This tool makes it easy to use different
objectives and filters. The high reproducibility of the system settings saves time and improves
your work.
 

Typical Application:

  • Ablation studies
  • Cell membrane analysis
  • Monitoring of surface trafficking
  • Nucleocytoplasmic shuttling
  • Protein diffusion studies
  • Region-specific Photoactivation
  • Acceptor photobleaching
  • Photoconversion studies

 

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