This optical component can be added on request to already installed CSU-W1 confocal scan heads. The existing
functionality of the original CSU confocal head remains. This enhancement offers even illumination of large cell areas and allows high-sensitivity imaging of living cells without the need for mathematical shading correction.
Laser-based Microscopy generally uses gaussian beams to achieve optimal focusing of the excitation light in the sample plane. However, this requires making a trade-off between excitation uniformity and intensity. The new Visitron VS-Homogenizer tackles this challenge by providing a flat intensity profile whilst maintaining optimal focusing of the laser beam in the sample plane.
Uniformity improvement to 5-15% depending on used microscope equipment and used microscope optics
Single mode fiber coupling with minimal power loss at pinhole
Maintains high signal to noise ratio of standard Yokogawa CSU-W1
No beam conditioning unit required
Flat Intensity Profile
Optimal Light Efficiency
An increasing number of live cell imaging applications requires the acquisition of large sample areas. Quantitative measurements over complete Slide Scans or Multi-Well Experiments benefit strongly from a uniform excitation intensity profile. The Visitron Homogenizer achieves this with minimal beam modifications for optimal light efficiency and signal-to-noise ratio.
13x13mm Field of View of a sCMOS camera and 63x/1.4 Oil Objective.
Scan Slide Acquisition of 6x8 images with Visitron Homogenizer.
Scan Slide Acquisition of 6x8 images with a standard Yokogawa CSU-W1 System.