The Total Internal Reflection Fluorescence (TIRF) technique is the ideal method for observations of cells close to the coverslip surface. By total reflection of the excitation light (typically laser) at the coverslip / medium interface, the fluorescence emission is limited to a very thin space in the vicinity of the glass surface. The resulting fluorescence images exhibits extremely high contrast and resolution with the possibility of real-time imaging of cell membranes, actin filament behavior or single molecule tracking.
The ORBITAL-600 system is very flexible microscope epi-illuminator. It can combine in one single output unit Ring-TIRF, 2D FRAP, Ablation with pulsed 355nm laser and Widefield. The Direct Laser Coupling (DLC) preserves the maximal laser power for FRAP/Ablation applications like Subcellular Nano-Surgery, Irradiation, DNA repair, Microstrokes or other Organelle destruction.
Point TIRF Ring TIRF
ORBITAL-500 Overview