Total Internal Reflection Fluorescence Imaging System

The TIRF technique is a special kind of wide-field fluorescence microscopy for measuring cells or SMC in an evanescent field without haze. By total reflection of the exciting light (typical Laser) at the coverslip/medium-interface the fluorescence excitation is limited to a very thin space in vicinity of the glass surface. The resulting fluorescence images exhibit extremely high contrast and resolution with the possibility of realtime Imaging of cell membrane or labeled single molecules SMC.

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VisiTIRF System & Principle

The VisiTIRF Fluorescence Imaging System from Visitron Systems is the ideal solution of this fluorescence technique for the analysis of finest structures. The flexibility of the VisiTIRF system and the use of scientific digital CCD camera systems with optimal resolution, speed and sensitivity allows the adaptation  of  customized application and configuration.
As fluorescence illumination we use different single or multiline Laser systems. The coupling of the Laser to the microscope is achieved  by a flexible optical fiber. Our special Visitron Systems TIRF condenser allows an easy adjustment of the laser beam to the correct illumination angle in the high aperture TIRF objective. A second optical fiber input allows the combination of TIRF and epi-fluorescence without moving any parts of the condensor. As epi- fluorescence illumination we have the choice to use the standard HBO lamp or the VisiChrome Polychromator system.

Dictyostelium specimen with gfp labeled tubulin Images courtesy by Prof. Gerisch, MPI Martinsried

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