Meta Imaging Series


Fluorescence ratio imaging involves the introduction of a fluorescent indicator into living cells to allow the monitoring of these cells through a microscope using a photodetector. In imaging, the detector is a sensitive digital or video camera.
Indicator dyes have been designed which shift their fluorescence excitation or emission spectrum upon binding the ions of interest. Images are obtained at two appropriate wavelengths, typically matching the absorption maxima at the high and low binding conditions.

By ratioing the intensities in the image at corresponding picture elements, a map can be constructed showing the local ion concentrations throughout the field of view. Since the monitoring process is nondestructive, image acquisition can be repeated frequently to trace out the time course of cellular responses.

Introduction To MetaFluor

MDCK cells loaded with fura-2 AM.
Imaged at the AQLM course in Woods Hole, MA. Upper right and left quadrant are 340nm and 380nm excitation images; lower left is a ratio map using IMD display. Lower right is a graph of selected cell ratio changes measured over time.


Designed for single or dual wavelength intracellular ion measurements, MetaFluor supports FURA-2, BCECF, INDO-1 and other common ratiometric indicators.
For easy viewing and analysis, MetaFluor provides simultaneous display of the original wavelengths, the ratio images, graphs of intensities, ratios, and ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image.

Two different ratiometric indicators can be imaged and measured at the same time. Computer controlled shutters minimize cellular exposure to the excitation illumination, reducing photo damage to your sample.

Fluo-3 labeled cells before and after stimulus.

Flexible Architecture

MetaFluor's flexible design offers the benefit of tailoring your system configuration to your specific application. This versatile architecture enables you to upgrade the system as new hardware, such as wavelength selectors and cameras, become available.
For example, your current application may require a video rate camera, but future applications may benefit from the high precision of a cooled CCD. MetaFluor's modular design makes it easy to take advantage of new technology as it becomes available.