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2D-VisiFRAP Realtime Scanner

Fluorescence Recovery After Photobleaching Imaging System

Fluorescent dyes show emission of one wavelength after they have absorbed light of short wavelength. Dyes, exposed to high intensity e.g. UV light react by photobleaching. The high intense light  renders the dyes unable to emit fluorescence. Fluorescence Recovery is based on this phenomenon and is typically used to measure the dynamics of molecular mobility or movement of fluorescent labeled molecules. It is also possible to measure the exchange of molecules between separate compartments of the cells.

2D VisiFRAP Software dialog

2D VisiFRAP block diagram

The 2D-VisiFRAP system from Visitron Systems GmbH is a microscope based imaging solution specially designed for fluorescence recovery or photoactivated studies. The system is based on a 2D galvanometer realtime scanner, a high sensitive CCD camera system, a research microscope like  Zeiss AxioObserver, Olympus BX/IX or Nikon E2000.
The 2D galvanometer scanner head is typically mounted on the epi fluorescence condenser. The laser light can be simple adapted via standard FC-connector. Customer requirements can be easily integrated due to the flexibility of the system. Realtime multi-point confocal technology can be easily combined to enhance resolution- and image quality.
Typically 405nm, 430nm, 457nm or 488nm laser-systems are used for bleaching multiple point or multiple area positioning is selectable by the new VS MetaImaging Software module which allow the positioning of the laser beam by two high speed galvanometer. Fast switching of the laser lines is done by the high speed VS-AOTF100 system or high speed shutter. The powerful and easy to use Meta-Imaging Software control the microscope and peripherals in combination with the camera system.

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