Fluorescence Recovery After Photobleaching Imaging System

All fluorescent dyes emit light of one wavelength after they have absorbed light of another wavelength. If a high intensity e.g. UV light illuminates the dyes, they react with photobleaching. The high intense light  renders the dyes unable to emit fluorescence. FRAP is working based on this phenomenon and is used typically to measure the dynamics of molecular mobility or movement of fluorescent labeled molecules. It is also possible to measure the exchange of molecules between separate compartments of the cells.

The VisiFRAP Fluorescence Imaging System from Visitron Systems GmbH is based on a high sensitive CCD camera system, typically an Axiovert-200 with motorized XY stage and a Laser-Slider system. Optional a Vt-Infinity multi-point confocal head can be used to enhance the image to confocal quality. All components are fully automatically controlled by the Visitron Software. Customer requirements can be easily integrated because of the flexibility of the system.

For the bleaching effect we typically use 405nm, 430nm, 457nm or 488nm Laser systems. The positioning of the selected  area for bleaching is done by the motorized XY stage. Single points / pixel areas as well as ROIs can be scanned. Preview and adjustment of the ROI could be done by an integrated Pilot IR Laser. Fast switching of the Laser lines is done by an AOTF or a high speed shutter. The Visitron Software controls the microscope and peripherals in combination with the camera system. Multidimensional 3D/4D control and analysis can be easily selected.

Get 1D-VisiFRAP brochure