The Dual-View utilizes a single beam splitter to split the incident beam from themicroscope into two independent beams. One beam contains all the
emission reflected offof the beam splitter; the other contains all the emission transmitted through the beamsplitter. Each of these emission channelsis projected onto half of the CCD array at
exactlythe same point in time. Simultaneous multichannel imaging is essential to achievequantitative emission ratiometric analysis.