BandView

Fully-automated image acquisition and analysis system for Karyotyping

What is G-Banding and Karyotyping?

G-banding is the treatment of chromosomes in the metaphase stage with trypsin (to partially digest the protein) and stain them with Giemsa. Each homologous chromosome pair has a unique pattern of g-bands, enabling recognition of particular chromosomes

A karyotype is an organized profile of a Human, Plant and Animal chromosomes. In a karyotype, chromosomes are arranged and numbered by size, from largest to smallest. This arrangement helps scientists quickly identify chromosomal alterations that may result in a genetic disorder.

BandView
BandView® is Applied Spectral Imaging's fully-automated image acquisition and analysis system for Karyotyping. You can either run it as an independent Karyotyping system and upgrade later to FISHView or HISKY or integrate it into your CytoLabView system. With the assistance of user input, ASI has created a user friendly, and accurate Karyotyping system.

G-, R-, Q- or DAPI-banding with BandView combines high-quality image processing with a complete working environment for the cytogeneticist

BandView brings you:

• Powerful automated karyotyping tools
• Superb image resolution
• Advanced enhancement algorithms
• Convenient image gallery to scan metaphases and karyotypes
• Multi-language database Case Data Manager (CDM) with link and reports
• Access to patient data statistics according to the parameters you choose
• Seamless upgrade to HISKY and FISHView (no need to change the camera, database or microscope)
• No loss of original image data during analysis
• Automated classification of brightfield or fluorescently stained metaphases
• Proprietary background subtraction enables automatic chromosome segmentation on sub-optimal metaphases
• Easy separation of touching and overlapping chromosomes
• Chromosome straightening without losing original image data
• ISCN 2005 Ideograms for 300,400, 550, 700 and 850 band resolution
• Ideogram editor to display breakpoints and translocations in the ideogram
• Robust same-case comparison tool to compare chromosomes in the same pair
• Cross-application comparison tool to compare band, FISH and HISKY analysis
• Dynamic karyotype table to fit any number of chromosomes

Acquisition Features - Image Capture

• High resolution images with 12-bit digital CCD-camera (1280x1024 pixels)
• Live-view image display throughout image acquisition
• User defined Region of Interest (ROI)
• Extremely low dark noise allowing for long exposure times
• Automatic exposure for brightfield, phase contrast and epi-fluorescence
• Easy graphic user interface for quick image acquisition
• Convenient image gallery to preview images before analysis
• MergeView - Unlimited joining of frames to create one image from multiple images of a sparse metaphase